We treated AML mice with vehicle or 50 mg/kg JQ1 daily for 5 days before enriching spleen-derived CD8+ T cells by magnetic bead sorting and profiling via s3-ATAC as previously described, with a few optimizations to achieve a cleaner chromatin accessibility signal on immune cell types (methods) [37, 45]. This evidence concerns the gene CD8A and acute myeloid leukemia.