ANXA5 and obesity due to melanocortin 4 receptor deficiency: circGlis3 overexpression in MIN6 cells resulted in a striking reduction in palmitate-induced apoptosis, as showed by Annexin V/PI staining and quantification of the cells displaying nuclei, suggesting that circGlis3 upregulation results in resistance to obesity-induced apoptosis of β-cells (Fig. 3j).