Based on this, and the fact that the gut modulates the metabolism and vice versa we evaluated the bacterial composition of the fecal gut microbiome in our PD mouse models (P479L rotenone experiment at day 32, Wt+R+E experiment at day 62, PARK2 vs Wt, PARK2+V vs PARK2+E, PARK2+V vs PARK2+R at day 32, and PARK2+R+E at day 54 experiment) using taxonomic marker gene sequencing (16S rRNA gene). The gene discussed is PRKN; the disease is Parkinson disease.