We further confirmed the differential effect of Sarm1 KO on the degeneration of ON segments proximal and distal to injury by comparing measures of neuropathy (pathology and axon loss) from the same ONs with mixed-effects analyses for the effect of location (distal versus proximal), genotype (wt versus Sarm1 KO), time, and their interactions (Fig. 7). This evidence concerns the gene SARM1 and neuropathy.