For lentivirus-mediated overexpression of MAGEA3 in osteosarcoma cells, full-length human MAGEA3 (NM_005362.3) was inserted into the pHBLV-CMV-MCS-3FLAG-EF1-ZsGreen-T2A-PURO lentiviral vector (Hanbio Biotechnology, Shanghai, China). The gene discussed is MAGEA3; the disease is osteosarcoma.