CD8A and osteosarcoma: To clarify the efficacy of γδ T-APCs in inducing antitumor responses of CD8+ T cells, we used osteosarcoma cell lines as target cells and performed a lactate dehydrogenase (LDH) assay and 7-AAD staining to determine the cytotoxic activity of CD8+ T cells cross-primed by γδ T-APCs.