For further validation, we evaluated the immune cells infiltration of 232 HCC samples from ICGC database with CIBERSORT algorithm (Figure S3A), and explored the correlation between NCF2 expression level and immune cells infiltration, it showed that M0 and M2 macrophages were positively correlated with NCF2 expression (Figure S3B,C), and M1 macrophages was negatively correlated with NCF2 expression (Figure S3D). The gene discussed is NCF2; the disease is hepatocellular carcinoma.