CD274 and cancer: Some of the U251 activated canonical pathways at 48 hpi include interferon signaling, role of pattern recognition receptors in recognition of bacteria and viruses, role of PKR in interferon induction and antiviral response and Th1 pathway, while some of the IPA predicted inhibited canonical pathways include PD-1, PD-L1 cancer immunotherapy pathway and coronavirus pathogenesis pathway (Figure 4).