The amount of rAAV-GFP transducing units could be amplified by a factor of up to 200 in a single step through co-infection with rAdV-M2-Tet-sRep and rAd-HSV-TK-Cap, although quite high MOIs of rAdV-M2-Tet-sRep (4000 to 8000 genomic particles per cell) were required for optimal yields (Figure 5C). The gene discussed is TKT; the disease is coinfection.