TKT and infection: In agreement with the data from the optimization experiments, the yields of purified rAAV-GFP vector fractions for the positive control (pTR-UF5/pDG co-transfection), the pTR-UF5 transfection followed by rAdV-M2-Tet-sRep/rAdV-HSV-TK-Cap co-infection, the rAdV triple infection and the rAAV-GFP amplification mediated by co-infection with the rAdV-M2-Tet-sRep/rAdV-HSV-TK-Cap combination (Figure 6A, first four bars) were all in a similar range of about 5.0 to 7.5 × 103 vector genomes/input cell (vg/cell, the so-called burst size).