To examine whether motor neuron autophagy plays a role in amyotrophic lateral sclerosis (ALS), Tian et al. generated a double transgenic (DTg) mouse model by crossing green fluorescent protein (GFP)-fused LC3 transgenic (LC3-Tg) mice with G93A mutant human Cu/Zn superoxide dismutase (mSOD1) transgenic (mSOD1-Tg) mice [186]. Here, SOD1 is linked to amyotrophic lateral sclerosis.