Notably, multiple antiviral proteins were also dramatically decreased in PAMs during the PRRSV-ADE infection, including ISG15, ISG20, IFIT1 (ISG56), IFIT2 (ISG54), IFIT3 (ISG60), IFIT5 (ISG58), OAS1, Mx1, RSAD2, TRIM22, TRIM25 and TRIM34, except for TRIM52. Here, ISG15 is linked to infection.