Studies utilising 3D porous chitosan-alginate scaffolds have also shown that the osteolytic PC-3 cell line did not demonstrate positive staining for mineralisation or express the bone matrix protein, osteocalcin, while these markers were present in samples cultured with the prostate cancer cells C4-2B and 22Rv1, which display osteoblastic behaviour [62]. Here, BGLAP is linked to prostate cancer.