Choi et al. established murine macrophage Raw264.7 cells expressing enhanced firefly luciferase (Raw/effluc) and murine colon cancer CT26 cells coexpressing Rluc and mCherry (CT26/Rluc-mCherry, CT26/RM) to monitor the migration of TAMs to tumor lesions and the effects of TAMs on tumor progression through bioluminescence imaging (BLI) and fluorescence imaging (FLI) [86]. This evidence concerns the gene DDX53 and malignant colon neoplasm.