The assembly of the IS by a trispecific T cell engager (TriTE) against EGFR, EpCAM, and CD3ε in comparison with two bispecific EGFRxCD3ε and EpCAMxCD3ε LiTEs has been also evaluated under the microscope by co-culturing Jurkat cells with colorectal cancer cells expressing EGFR and EpCAM. This evidence concerns the gene EGFR and colorectal cancer.