TM4SF4 and hepatocellular carcinoma: In the study, when the TM4SF4 gene was silenced using adenovirus-delivered siRNA targeting TM4SF4 (AdSiTM4SF4) in QGY-7701 and SMMC-7721 HCC cells, the endogenous TM4SF4 expression was significantly inhibited, consequently effectively reducing cell growth and colony formation.