These results suggest that high levels of proteins as well as the MCo-4 and MCoCC-2 peptides in the seed extracts are likely responsible for the cytotoxic effects observed on the melanoma cells, most likely through triggering the TNFR1/NF-kB and BRAF/MEK1/Nrf2 induced cell death pathways. The gene discussed is TNFRSF1A; the disease is melanoma.