MΦ infected with 10 MOI O. tsutsugamushi also displayed significantly high expression of Il12p40, Tnf, and Il27p28. While 0.1 μM BAY had small (Il27p28) or virtually no effect (Il12p40 and Tnf), treatment with 1.0 μM BAY led to over 80% reduction in all tested cytokines in infection. The gene discussed is TNF; the disease is infection.