Administration of G. thunbergia extract of leaves at a dose of (200 mg/kg) and rutin (75 mg/kg) could prevent the development of NAFLD in HFD-fed mice through decreasing the oxidative stress mediated by β-oxidation of fatty acids, and decreasing inflammatory steatosis development through downregulation of the CYP2E1–cJNK apoptotic signaling pathway. This evidence concerns the gene CYP2E1 and metabolic dysfunction-associated steatotic liver disease.