The results from four independent models in our collective studies on N. mitis, including cAMP-dependent Cl− channel, TMEM16A (Ca2+-activated Cl− channel) [18], CO-induced diarrhoea, and the isolated tissue technique (organ bath) brought to light the antisecretory and antimotility potential of N. mitis, thereby giving credence to its traditional usage in diarrhoea therapy, and therefore validating its antidiarrhoeal activity. The gene discussed is ANO1; the disease is Diarrhea.