Matthew et al. [25] investigated the role of BSHs encoded by Lactobacillus acidophilus and found that BA-type and bile salt hydrolases (BSHs) substrate preference affect the growth of L. acidophilus in vitro and in vivo, and that Lactobacillus acidophilus-encoded BSHs are not used to promote colonization and detoxify bile, but rather their enzymatic preference and the inherent chemical characteristics of BAs determine their toxic effects on bacterial growth; BSHs act as probiotic colonization promoters and intervene to treat disease by lowering serum cholesterol or during obesity [27,28,29]. Here, CFB is linked to obesity due to melanocortin 4 receptor deficiency.