The top five mutated genes (AAAS, ACVR1B, ACVRL1, ADAMTS12, and ADCY2) between the ARHGAP21 changed group and unchanged group were determined and indicated in Figure 3D. The AAAS, ACVR1B, ACVRL1, and ADAMTS12 had a higher level of expression in CCA tissue than that in para-carcinoma tissue (Figure 3E). This evidence concerns the gene ADAMTS12 and cholangiocarcinoma.