The findings showed that E-cadherin negative expression was significantly correlated with lymph node metastasis (ORs = 3.21, 95% CI = 1.98–5.20), tumor node metastasis stage of thyroid cancer (ORs = 4.85, 95% CI = 2.86–8.25) and negatively correlated with differentiation (ORs = 0.25, 95%CI = 0.07–0.82), thus, supporting the results of the present study. The gene discussed is CDH1; the disease is thyroid cancer.