Singh and colleagues (2015) demonstrated the implication of ALDH1B1 in different CSC-related cellular signaling pathways, including Wnt/β-catenin, Notch, and PI3K/Akt in CRC [145], and ALDH1B1 shRNA in the colon adenocarcinoma SW480 cell line, led to the downregulation of various proteins, such as LEF1, C-Myc, JAG1, c-Notch1, Akt, PI3K, FABP5, and MMP2 [145]. This evidence concerns the gene AKT1 and colon adenocarcinoma.