Some biomarkers that were up-regulated in experiment series 3 overlapped between females (F) and males (M) in CKD: APOA1 (up), ANGPT2 (up), C5 (up), CFD (up), GH1 (up), ICAM1 (up), IGFBP2 (up), IL8 (up), KLK4 (up), MMP9 (up) and SPP1 (up). The gene discussed is SPP1; the disease is chronic kidney disease.