MTOR and neoplasm: Immunoblotting analysis was performed on the lysates of tumor mammary pads to analyze the levels of metabolic and proliferation markers such as p-AMPKα and p-mTOR, a marker of lipolysis of lipid droplets (p-Perilipin 1), immune cells (CD4, CD8, and macrophages F4/80), and inflammatory markers (TNFα, IFNƳ, and IL-6) (Figure 5).