To examine whether Rac1 regulates the migration of HCC cells in hypoxic conditions, we confirmed efficient siRNA knockdown of Rac1 by Western blot in Hep3B, SNU499, and Huh7 cells (Figure 3A and Figure S1), and right after that, we performed the wound healing assay and transwell migration assay using Rac1 siRNA-transfected HCC cells. Here, RAC1 is linked to hepatocellular carcinoma.