Given that inhibition of Rac1 repressed the expression of PAK1 and EMT-related markers including Snail, which is suggested to be related to metastasis and invasion in Hep3B cells, we investigated whether Rac1 modulates migration and invasion of HCC cells such as Hep3B, SNU499, and Huh7. The gene discussed is PAK1; the disease is hepatocellular carcinoma.