Immunoblots of different proteins of the Akt/mTOR and MEK/ERK pathways were used to assess proper drug action, as already previously established [14], and to determine molecular differences in DLD-1 colon cancer cells grown in 2D and 3D culture upon treatment with selected dual and triple inhibitor combinations (Figure 1F and Figure S1C). This evidence concerns the gene AKT1 and colonic neoplasm.