To test the in vivo the impact of targeting proNGF on GBM tumor growth, we used a xenograft GBM mouse model in which human U87MG cells (MGMT methylated) were injected subcutaneously between the shoulder blades of BALB/c nude mice, along with anti-proNGF antibody alone or in combination with TMZ. This evidence concerns the gene MGMT and neoplasm.