Moreover, TLR4 protein expression on neutrophils determined by flow cytometry was upregulated, driving dependent proinflammatory cytokine, tumor necrosis factor alpha, IL-8 increase, and decreased anti-inflammatory IL-10 release at HUS onset compared with patients with enterohemorrhagic Escherichia coli (EHEC) diarrhea, and healthy children. The gene discussed is TLR4; the disease is hemolytic-uremic syndrome.