The mRNA-splicing major and axon guidance pathways showed the highest number of antigens, which were more highly reactive in GBM-post, as compared to the seroreactivities of healthy individuals (contrast (b)): of 16 antigens found to be higher reactive in GBM-post, and present in the mRNA-splicing major pathway, SART1, PQBP1, RBM17 and POLR2A in healthy tissue, and 8 antigens (U2AF1, EFTUD2, HNRNPH1, HNRNPL, PRPF40A, TRA2B, CTNNBL1 and LSM4) were more strongly expressed in GBM tissue. This evidence concerns the gene POLR2A and glioblastoma.