MPO and Autoimmunity: To confirm the increased MPO autoimmunity and resultant kidney injury was TLR9 specific and reproducible, we utilized mice deficient in TLR9 (Tlr9−/−) to generate Tlr9−/− dendritic cells and compare to TLR9 stimulated MPO pulsed TLR9 intact C57BL/6 WT dendritic cells.