Figure 3A shows representative line-scan images of caffeine-evoked Ca2+ transients in each experimental group. Independently of genotype, the amplitude of Ca2+ transients induced by caffeine application were significantly lower in those animals with AKI compared to their corresponding controls (p < 0.001 vs. +/+ and p < 0.05 vs. +/kl, Figure 3B), indicating that partial klotho deficiency did not further decrease SR Ca2+ load to those observed under AKI conditions. The gene discussed is KL; the disease is acute kidney injury.