To quantify the antitumor effect of HPF on melanoma cells, a sulforhodamine B (SRB) viability assay was carried out on SK-Mel-28, A375 and FO-1 BRAF-mutated melanoma cells and on P53-mutated MeWo cells after 24, 48 and 72 h of treatment with 1, 2, 3, 4 and 5 μM HPF. Here, TP53 is linked to melanoma.