Cardiac overexpression of skd in transgenic (α-myosin heavy chain (αMHC)-MED13-TG or MED13-cTG) mice inhibits the binding of RNA polymerase II (Pol II) to MED, thereby inhibiting the transcription and expression of target genes involved in cardiac energy metabolism, improving insulin sensitivity and increasing energy expenditure, thereby preventing HFD-induced obesity [66]. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.