To further determine the potential effect of EPCAM on regulating tumor initiation in cervical cancer cells, EPCAMhigh and EPCAMlow cells were subcutaneously injected into NOD/SCID mice at limited dilutions with 10, 100, 1000, and 10,000 cells, respectively, and the tumor volume and incidence were monitored. This evidence concerns the gene EPCAM and neoplasm.