To study the mRNA distribution between the nucleus and cytoplasm, confocal images of 14 regions of interest (ROIs) from seven individual subjects and greater than 1400 cells (>600 from benign glands and low-grade cancer and >800 from high-grade cancer) for ANO7 and PPIB were analyzed to assess the nuclear to cytoplasmic ratio of the transcripts. This evidence concerns the gene PPIB and cancer.