SOD1 and amyotrophic lateral sclerosis: Although the effects of H2O2 are largely dependent upon the cell type used, to mimic the massive oxidative stress conditions reported in ALS patients, SH-SY5Y cells overexpressing the normal SOD1 enzyme (WT), or the mutated form (SOD1G93A), were treated with increasing concentrations of H2O2 (50–200 μM) for 24 h.