ABCB1 and non-small cell lung carcinoma: In this regard, calcein AM is a well-known substrate of ABCB1 [20,21,22], and using a functional test based on measurement of its intracellular accumulation [23], we could demonstrate that AIF-1 significantly induced calcein accumulation in NSCLC cellular models, an effect likely related to the inhibition of the calcein efflux process mediated by the membrane pump.