We also detected the levels of Th1 cell-related factors (IL-17 and IFN-γ), Th2 cell-related factors (IL-4, IL-5 and IL-13) and other factors associated with allergic symptoms, such as histamine, chitinase-3-like protein 1 and specific antibodies IgE, IgG1 and IgG2a, to verify whether the mice presented allergies or tolerance. This evidence concerns the gene IFNG and allergic disease.