For example, after negative predictions by several available machine learning-based computational tools used to explore the splicing effect of missense variants in patients with muscular dystrophy, limb-girdle, and autosomal recessive 1 (LGMDR1; OMIM: #253600), eight out of twenty-one deep exonic missense variants in CAPN3 were shown to affect splicing using minigene assays [37] (Table 1). This evidence concerns the gene CAPN3 and muscular dystrophy.