We assessed the effects of various shipping media (Hank’s Balanced Salt Solution (HBSS), University of Wisconsin solution (UW), and Histidine-Tryptophan-Ketoglutarate solution (HTK)) and dissociation times (Fresh, 24 h, and 48 h) on organoid viability, growth rate, LGR5+ and TROY+ stem cell populations, and anti-cancer drug response. Here, LGR5 is linked to cancer.