Ovarian cancer CAFs were characterized by negative expression of cell type markers, including leucocyte common antigen CD45, macrophage marker CD68, endothelial marker CD31, and epithelial marker EpCAM with parallel positive expression of fibroblast markers, SMA, FAP, S100A4, and TE-7 (flow cytometry, qRT-PCR, Western blot, and ICC). Here, S100A4 is linked to ovarian cancer.