Our data revealed that the mRNA expression (p < 0.01) and protein levels (p < 0.01) of TLR2 and TLR4 were significantly up-regulated in bovine mastitis mammary gland tissues, suggesting that bovine mammary gland tissue initiates different innate immune regulatory pathways to respond to pathogenic infections [22,54]. The gene discussed is TLR2; the disease is mastitis.