Considering the poised genetic composition from a single cell line may cause the biased selection of OTUD7B inhibitors, as well as the regulation of Akt-pS473 through OTUD7B-independent mechanisms, we further tested the effects of 10 common hits identified from both Akt-pS473 ELISA and Western blotting (Figure 1I) in additional NSCLC cell lines, including A549 (Figure 2A and Figure S1A), H358 (Figure 2B), H520 (Figure 2C and Figure S1B) and H1299 (Figure 2D and Figure S1C) at a single dose of 10 μM for 16 h. The gene discussed is AKT1; the disease is non-small cell lung carcinoma.