In our study, we reveal that the knockdown of LNC EBLN3P induced by carbon ion irradiation could inhibit cell proliferation and induce apoptosis and radiosensitivity of NSCLC cells through the down-regulation of TNPO1, demonstrating that TNPO1 may be a key factor functioning in the radiobiological effects of heavy ions. Here, TNPO1 is linked to non-small cell lung carcinoma.