To explore the biological functions of circZBTB46 in AML, two siRNAs targeting the backsplice junction (BSJ) region of circZBTB46 were designed, and they specifically inhibited circZBTB46 expression (Figure 3A) and did not change the levels of ZBTB46 mRNA in AML cells (Figure S3A). The gene discussed is ZBTB46; the disease is acute myeloid leukemia.