In refractory AML, WT1 (p < 0.001) was more frequent, meanwhile relapse AML exhibited more mutations in RUNX1 (p = 0.037), DNMT3A (p = 0.018), IDH1 (p = 0.017) and WT1 (p < 0.001). This evidence concerns the gene DNMT3A and acute myeloid leukemia.