In conclusion, it should be noted that use of the locus-specific NGS method not only provides data on the methylation of individual molecules of the RNF219, KIAA1539, and GSTP1 genes in the tcfDNA of patients with prostate cancer and healthy donors, but also enables differentiation of both studied groups of patients with 100% sensitivity and specificity. Here, GSTP1 is linked to prostate cancer.