Immune-related factors included the neutrophil/lymphocyte ratio (N/L ratio), spontaneous IFN-γ, TNF-α, and IL-10 released from PBMCs, as well as WT1-332-specific IFN-γ, TNF-α, and IL-10 released from PBMCs in all patients with malignant glioma and WT1-235-specific IFN-γ and IL-10 released from PBMCs in patients with HLA-A*24:02. Here, WT1 is linked to malignant glioma.