To explore the mechanism underlying the reduction of the proliferative potential of ESCC cells by IL-7R silencing, the expression levels of apoptosis-related protein Caspase-3 and Caspase-8, necroptosis-related protein RIP and MLKL, and cell cycle-related protein Cyclin D1 were examined by Western blot analysis (Figures S5 and S7J). The gene discussed is MLKL; the disease is esophageal squamous cell carcinoma.