The results revealed that TNNT1, HOXC6, and SPHK1 were expressed markedly higher in CRC cell lines (HCT116, SW480, and SW620) than in normal cell lines, HES4 was highly expressed in HCT116 and SW620 cell lines, and SFRP2 was significantly lower in colorectal cancer cell lines (HCT116, SW480, and SW620) (Figure 10). This evidence concerns the gene TNNT1 and colorectal cancer.